Summary
Type: SPE
Application #: 301420
Catalog#: 730077 - SPE Cartridges, Chromabond, SA, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
Force or aspirate 1 column volume n-hexane each through the SA and the ALOX
columns; then pour 1 ml n-hexane into the ALOX column and place the SA column
onto the ALOX column using the adaptor
Sample application:
Force or aspirate 0.5 ml of the clear supernatant from the sample pretreatment
through both columns; collect the eluate
Column washing:
Force or aspirate 3 x 0.5 ml n-hexane through the columns; collect the eluates
Elution:
Remove the SA column and elute the ALOX column with 5 ml cyclohexane/
dichloromethane (85 : 15, v/v) – combine the eluate with the other eluates and
concentrate
Authors: LC Department
Source: Macherey-Nagel